VCS is running on all three nodes, and the configuration is closed. The administrator runs "hastop -all -force", manually edits the main.cf on NodeA, and runs hastart on NodeA, but VCS does not start. Which command should they run to identify the problem?
hasys -force NodeA
Which tool is required to collect information about a cluster to send to Symantec Support?
VEA GUI and VCS GUI
Vertias cluster server simulator
In a two-node VCS cluster, all heartbeat links simultaneously failed when a quad-ethernet card that supports these networks went down on one of the servers. What might this cause?
The GAB membership will go into a jeopardy state to prevent any service groups from failing over.
The violation trigger script will be called to shutdown one of the servers to minimize the risk of data corruption.
Thecluster will partition and create a split-brain condition which may result in data corruption.
The service groups will be auto-disabled until one or more of the heartbeat links resumes.
For what two purposes should the VCS Simulator tool be used? (Select two.)
To replay and simulate the event captured in the VCS log files for investigating problems
To enable offline testing of VCS configurations
To pre-stage VCS configurations before they are rolled-out to the cluster nodes
To simulate servers and arrays used in a VCS configuration
To simulate VCS configurations for testing custom agents and scripts
Which of the following enables you to test the O/S and application configuration of a node by bringing up the application on an inactive node without stopping the application on the primary node ordisrupting user access?
HA Fire Drill
Fire Drill using snapshots
A customer wants to deploy two production applications, each with its own corresponding test application and wants to prevent production applications running on the same system as test applications. What should be the best practice recommendation?
Deploy all four applications in one single cluster and use offline local dependencies
Deploy two clusters with each containing a production application and its corresponding test application and use offline local dependencies
Deploy two clusters with each containing a production application and its corresponding test application and use system limits and service group prerequisites
Deploy two clusters, one containing both production applications and one containing both test applications
four.6. Immunofluorescence microscopy
For immunofluorescence microscopy, cells seeded on coverslips
have been cultured in 24-smartly dishes. After remedy with acidic
medium at pH 6.0 for twenty-four h, cells have been fastened in four% paraformalde-
hyde (Sigma) for 15 min, washed with PBS, after which permeabi-
lized with 0.1% Triton-X-a hundred(Sigma) for 15 min. The basic anti-
ASICs (ASIC1, ASIC2, ASIC3, ASIC4) antibody (1:250 dilution, Santa
Cruz; Alomone Labs) became added into the cells and incubated at
room temperature for two h and Cy3-conjugated anti-goat anti-
bodies (1:500; Beyotime Institute of Biotechnology) have been used
for 1 h. eventually, after the cells had been incubated with 4′,6-diamidino-
2-phenylindo le: DAPI (3 μg/ml, Sigma) for 10 min, the slides were
established with Citiflour (C itiflour Ltd.). The cells were then
visualized beneath a Leica TSC ST2 confocal microscope.
All facts are expressed as t-check. Two-neighborhood comparisons had been
performed the use of student's t-test. numerous-neighborhood comparisons
have been carried out the use of one-method evaluation of variance and
Fisher's least large difference (equal variances assumed) or
Dunnett's T3-check (equal variances now not assumed). A probability
lower than 0.05 changed into regarded statistically big.
we're grateful to Dr. Jin Tao of Soochow institution for his
tips, generosity and fabulous aid with facilities
and reagents. We also thank professor Tian-le Xu in Labora-
tory of Synaptic Physiology, Institute of Neuroscience of
chinese Academy of Sciences. We additionally thank Xing-shun Xu
for essential analyzing of the paper. This work is supported through
promises from the Jiangsu Province basis of China.
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table 1 –Primers utilized in PCR and size of products.
sense primer Antisense primer size (bp)
ASIC1a GCCCACATCTTCTCCTAT CTTGGTGACGTGGTGATA 212
ASIC1b CGAAGCAGGCATCAAAGT CGAAGAAATCCGAGTCCAT 179
ASIC2a GCAACCTGACACGCTACAA GGCAGCAACTTCATACGC 191
ASIC2b ACCTGACACGCTACAACAA GCAACTTCATACGCCTTC 184
ASIC3 ATACAACCGCAGCGAGTC TCTTCCTGGAGCAGAGTGTT 246
ASIC4 TCGCTACCCAGAGCCTGACA CTCTGAGGATCTGCATTGAAGGTG 168
β-actin TCAGGTCACTATCGGCAAT AAAGAAAGGGTGTAAAACGCA 432
86 mind analysis 1396 (2011) 77– 87